Patent Number:
10,030,233
Title:
Modified P450 reductase with N-terminal deletions and endoplasmic reticulum retention tag
Abstract:
The present invention provides an isolated or recombinant polypeptide comprising or consisting of a modified P450 reductase which lacks N-terminal amino acids relative to the corresponding wild type P450 reductase and comprises an epitope tag comprising the sequence HDEL or KDEL. The modified P450 reductase, when co-expressed with a cytochrome P450, increases the activity and/or expression of the cytochrome P450 compared to the activity and/or expression of the cytochrome P450 when co-expressed with the wild type P450 reductase.
Inventors:
Chaudhuri; Bhabatosh (Leicester, GB)
Assignee:
DE MONTFORT UNIVERSITY
International Classification:
C12N 9/02 (20060101)
Expiration Date:
2022-07-24 0:00:00