Patent Number: 10,030,233

Title: Modified P450 reductase with N-terminal deletions and endoplasmic reticulum retention tag

Abstract: The present invention provides an isolated or recombinant polypeptide comprising or consisting of a modified P450 reductase which lacks N-terminal amino acids relative to the corresponding wild type P450 reductase and comprises an epitope tag comprising the sequence HDEL or KDEL. The modified P450 reductase, when co-expressed with a cytochrome P450, increases the activity and/or expression of the cytochrome P450 compared to the activity and/or expression of the cytochrome P450 when co-expressed with the wild type P450 reductase.

Inventors: Chaudhuri; Bhabatosh (Leicester, GB)

Assignee: DE MONTFORT UNIVERSITY

International Classification: C12N 9/02 (20060101)

Expiration Date: 2022-07-24 0:00:00