Patent Number: 6,165,469

Title: Recombinant Entamoeba histolytica lectin subunit peptides and reagents specific for members of the 170 kD subunit multigene family

Abstract: The adhesin 170 kDa subunit of Hm-1:IMSS strain of Entamoeba histolytica is encoded by a gene family that includes hgl1, hgl2 and a previously undescribed third gene, hgl3, for which the DNA and protein sequences are disclosed. All three of these heavy subunit genes were expressed in the amebae. Methods and reagents (both nucleic acid and immunological) which are specific for each of the genes, as well as reagents which detect common regions of all three hgl genes or their nucleic acid or protein products, are disclosed. Recombinantly produced heavy chain subunit of E. Histolytica Gal/GalNAc adherence lectin or an epitope-bearing portion thereof may be used as antigen in serological analysis for E. histolytica infection or as an immunogen for protection against infection. Recombinant production in procaryotic systems provides antigens or immunogens which are immunologically reactive.

Inventors: Mann; Barbara J. (Charlottesville, VA), Petri; William A. (Charlottesville, VA)

Assignee: University of Virginia

International Classification: C12Q 1/68 (20060101); A61K 039/002 ()

Expiration Date: 12/26/2013