Patent Number: 6,165,496

Title: Keratin-based sheet material for biomedical applications and method of production

Abstract: A sheet wound dressing formed of cross linked keratin. An insoluble, largely Beta keratin fraction from human hair is acidified to a low pH, preferably less than about 3, which partially solubilizes the keratin by weakening hydrogen bonds. The suspension is added to base, such as ammonium hydroxide, forming a slurry. The slurry is cast directly onto a flat surface, allowing the re-formation of cross-links including hydrogen bonds and disulfide bonds. The resulting cross-linked keratin sheet can be used as a sheet wound dressing or as a scaffolding for growth of cells. The insoluble keratin can be derived from human hair which is washed, rinsed, dried, chopped and treated with peracetic acid to break some accessible disulfide linkages. The treated hair is filtered, rinsed, dried, and ground into a keratin powder. The keratin powder is suspended in a mixture of ammonium hydroxide and ammonium thioglycollate and heated sufficiently to dissolve the soluble keratin fraction, followed by cooling and centrifugation to concentrate the insoluble, largely Beta keratin fraction.

Inventors: Timmons; Scott F. (San Antonio, TX), Blanchard; Cheryl R. (San Antonio, TX), Smith; Robert A. (Jackson, MS)

Assignee: Keraplast Technologies, Ltd.

International Classification: A61K 38/39 (20060101); A61L 15/32 (20060101); A61L 15/16 (20060101); A61L 27/60 (20060101); A61L 27/22 (20060101); A61L 27/00 (20060101); A61L 27/34 (20060101); A61L 26/00 (20060101); A61L 31/10 (20060101); A61L 31/04 (20060101); A61L 31/08 (20060101); C08H 1/06 (20060101); C08H 1/00 (20060101); A61K 009/00 (); A61K 009/70 (); A61K 038/00 (); A61F 013/00 (); A61L 015/16 ()

Expiration Date: 12/26/2017