Patent Number: 6,165,715

Title: Expression systems

Abstract: The invention relates to new expression systems and in particular to an expression system in which a gene of interest is expressed at an optimal level. The invention provides a recombinant expression vector comprising a gene of interest and a selectable marker gene, wherein the selectable marker gene is arranged downstream of the gene of interest and a stop codon associated with the gene of interest is spaced from a start codon of said selectable marker gene at a distance which is sufficient to ensure that translation reinitiation is required before said selectable marker protein is expressed from the corresponding mRNA. Examples of such expression systems are vector viral packaging cell lines and a number of preferred cell lines have been identified.

Inventors: Collins; Mary Katherine Levinge (London, GB), Weiss; Robin Anthony (London, GB), Takeuchi; Yasuhiro (London, GB), Cosset; Francois-Lois (Lyons, FR)

Assignee: Cancer Research Campaign Technology Limited

International Classification: C12N 15/67 (20060101); C12N 15/867 (20060101); C12Q 001/68 (); C12N 005/10 (); C12N 015/86 (); C12N 015/63 ()

Expiration Date: 12/26/2017