Patent Number: 6,165,718

Title: Method for in vivo production of a mutant library in cells

Abstract: A method for in vivo production of a library in cells comprising a multitude of mutated genetic elements, wherein an error-prone polymerase is used in each ancestral cell to replicate all or a part of a genetic element independently of the host chromosomal replication machinery. The genetic element comprisesi) an origin of replication from which replication is initiated,ii) optionally a genetic marker, e.g. a gene conferring resistance towards an antibiotic,iii) a gene encoding the polypeptide of interest.Also methods for the generation of a DNA sequence encoding a desired variant of a polypeptide of interest, and for the determination of such a DNA sequence are described.

Inventors: Borchert; Torben Vedel (Jyllinge, DK), Ehrlich; Stanislas Dusko (Paris, FR)

Assignee: Novo Nordisk A/S Novo Alle

International Classification: C12N 15/10 (20060101); C12Q 001/68 (); C12N 015/70 (); C12N 015/63 (); C12N 009/10 (); C12N 009/14 ()

Expiration Date: 12/26/2013