Patent Number: 6,165,722

Title: Representations of bimolecular interactions

Abstract: A method of preparing a bimolecular interaction library for a first biological unit and for a second biological unit, each of the first and second biological units having a corresponding genetic material, the method comprising the steps of: (a) preparing a first fragment from the genetic material corresponding to the first biological unit; (b) preparing a first phage library having a first selection marker with the first fragment, such that a first peptide is displayed by the first phage library; (c) preparing a second fragment from the genetic material corresponding to the second biological unit; (d) preparing a second phage library having a second selection marker with the second fragment, such that a second peptide is displayed by the second phage library; (e) mixing the first phage library and the second phage library; and (f) co-selecting co-selected phages from the first phage library and from the second phage library by the first selection marker and the second selection marker when a selection process yields a positive result, such that the selection process yields the positive result only when the first peptide and the second peptide interact, and such that the bimolecular interaction library is formed from the co-selected phages.

Inventors: Gershoni; Jonathan M. (Rehovot, IL), Enshel; David (Givatayim, IL)

Assignee: Ramot University Authority for Applied Research & Industrial Development Ltd.

International Classification: A61K 49/00 (20060101); C07K 14/00 (20060101); C12Q 1/68 (20060101); C12N 15/10 (20060101); G01N 33/68 (20060101); G01N 33/569 (20060101); A61K 39/00 (20060101); C12Q 001/70 (); C12Q 001/68 (); C12P 021/00 (); C12P 021/08 ()

Expiration Date: 12/26/2017