Patent Number: 6,165,729

Title: Electrochemiluminescent reaction utilizing amine-derived reductant

Abstract: Electrochemiluminescent assay methods for determining an analyte of interest, which methods include forming one or more compositions which include, in the aggregate, (a) a sample to be tested for the analyte of interest, (b) a component capable of specifically binding with the analyte, (c) a label reagent which, when oxidized, is capable of electrochemiluminescence, (d) an amine which, when oxidized, forms a reducing agent, and (e) an electrolyte solution; subjecting such a composition containing the sample to conditions sufficient for specific binding to occur between the analyte of interest, if present, and one or more of the other components in the composition; thereafter, a composition containing the label reagent and amine, and reflecting the outcome of applying the aforementioned binding conditions, is exposed to conditions, such that both the label reagent and the amine are oxidized, the amine forms a reducing agent which interacts with the label reagent, electrochemiluminescence occurs, and the luminescence emitted from the label reagent is used to determine the analyte of interest.

Inventors: Leland; Jonathan Kent (Laurel, MD), Powell; Michael Joseph (Danville, CA)

Assignee: Hyperion Catalysis International, Inc.

International Classification: C07D 405/14 (20060101); C07D 213/00 (20060101); C07F 15/00 (20060101); C07D 213/48 (20060101); C07D 405/00 (20060101); C07H 21/00 (20060101); C07D 213/55 (20060101); C07D 213/22 (20060101); C07F 13/00 (20060101); C07D 401/12 (20060101); C07D 401/00 (20060101); C07D 473/00 (20060101); C07D 473/08 (20060101); C12Q 1/68 (20060101); C12Q 1/04 (20060101); G01N 33/76 (20060101); G01N 33/58 (20060101); G01N 33/569 (20060101); G01N 33/533 (20060101); G01N 33/74 (20060101); C12Q 001/70 (); G01N 033/53 (); G01N 021/76 (); G01N 033/566 ()

Expiration Date: 12/26/2017