Patent Number: 6,165,730

Title: Hepatitis C virus peptides obtained from the NS4 coding region and their use in diagnostic assays

Abstract: The etiological agent responsible for non-A, non-B hepatitis has been identified and termed hepatitis C virus (HCV). The HCV genome is a linear, positive-stranded RNA molecule with a length of approximately 9,400 nucleotides. With the exception of rather short untranslated regions at the termini, the genome consists of one large, uninterrupted, open reading frame encoding a polyprotein of approximately 3,000 amino acids. This polyprotein has been shown to be cleaved co-translationally into individual viral structural and non-structural (NS) regions. The structural protein region is further divided into capsid (Core) and envelope (E1 and E2) regions. The NS regions are divided into NS-1 to NS-5. The present invention is directed toward peptides derived from the HCV NS4 region that contain immunologically important epitopes. A series of biotinylated peptides were prepared by solid phase peptide synthesis and these peptides have proven useful for the detection of HCV-specific antisera.

Inventors: De Leys; Robert (Grimbergen, BE)

Assignee: N.V. Innogenetics S.A.

International Classification: C07K 14/005 (20060101); C07K 14/18 (20060101); C07K 14/16 (20060101); G01N 33/569 (20060101); G01N 033/53 ()

Expiration Date: 12/26/2017