Patent Number: 6,165,745

Title: Recombinant production of immunoglobulin-like domains in prokaryotic cells

Abstract: Disclosed are recombinant vectors encoding immunoglobulin-like domains and portions thereof, such as T-cell variable domains, antibody Fc-hinge fragments, subfragments and mutant domains with reduced biological half lives. Methods of producing large quantities of such domains, heterodimers, and fusion proteins following expression and secretion by prokaryotic host cells are also reported. Described are single chain T-cell receptors, which are folded into .beta.-pleated sheet structures similar to those of immunoglobulin variable domains; antibody Fc and Fc-hinge domains, which have the same in vivo stability as intact antibodies; and domains engineered to have reduced half lives.

Inventors: Ward; E. Sally (Dallas, TX), Kim; Jin-Kyoo (Irving, TX)

Assignee: Board of Regents, The University of Texas System

International Classification: A61K 47/48 (20060101); C07K 14/435 (20060101); C07K 14/74 (20060101); C07K 14/725 (20060101); C07K 14/735 (20060101); C07K 14/73 (20060101); C07K 14/705 (20060101); C07K 16/00 (20060101); C12P 021/06 ()

Expiration Date: 12/26/2013