Patent Number: 6,166,230

Title: Sterol extraction with polar solvent to give low sterol, high triglyceride, microbial oil

Abstract: The present invention relates to a process of treating an oil, the process comprising contacting the oil with a polar solvent to extract at least one compound that is soluble in the solvent, and then separating the solvent containing the compound from the so treated oil. The oil is microbially derived, and extracted either from a fermentation broth or a filtrate thereof using hexane. The compound to be extracted is usually a sterol or a diglyceride. The solvent is ethanol having up to 5% water. The oil can contain a polyunsaturated fatty acid such as C18, C20 or C22 .omega.-3 or .omega.-6 fatty acid, such as arachidonic acid.

Inventors: Bijl; Hendrik Louis (Vlaardingen, NL), Wolf; Johannes Hendrik (Delft, NL), Schaap; Albert (Barendrecht, NL)

Assignee: Gist-brocades B.V.

International Classification: A23D 9/007 (20060101); A23D 9/00 (20060101); A23K 1/16 (20060101); A23L 1/30 (20060101); A61K 8/96 (20060101); A61K 8/99 (20060101); A61K 31/21 (20060101); A61K 31/232 (20060101); C11B 3/00 (20060101); C12P 7/64 (20060101); C07C 053/00 ()

Expiration Date: 12/26/2017