Patent Number: 6,166,853

Title: Method and apparatus for three-dimensional deconvolution of optical microscope images

Abstract: An adaptive structure of a Wiener filter is used to deconvolve three-dimensional wide-field microscope images for the purposes of improving spatial resolution and removing out-of-focus light. The filter is a three-dimensional kernel representing a finite-impulse-response (FIR) structure requiring on the order of one thousand (1000) taps or more to achieve an acceptable mean-square-error. Converging to a solution is done in the spatial-domain and therefore does not experience many of the problems of frequency-domain solutions. Alternatively, a three-dimensional kernel representing an infinite-impulse-response (IIR) structure may be employed. An IIR structure typically requires fewer taps to achieve the same or better performance, resulting in higher resolution images with less noise and faster computations.

Inventors: Sapia; Mark A. (Canton, CT), Schaff; James C. (Cheshire, CT), Greenshields; Ian R. (Mansfield Center, CT), Loew; Leslie M. (W. Hartford, CT), Morgan; Frank R. (W. Hartford, CT)

Assignee: The University of Connecticut

International Classification: G02B 21/00 (20060101); G06E 3/00 (20060101); G06T 5/20 (20060101); G02B 027/46 (); G06K 009/76 (); G06K 009/40 ()

Expiration Date: 12/26/2013