Patent Number: 6,167,710

Title: Ice seeding apparatus for cryopreservation systems

Abstract: An apparatus for ice seeding during cryopreservation of biological samples such as cells, harvested tissues, and cellular biological constructs such as culture tissues equivalents wherein ice seeding is performed by discharging a liquefied or chilled gas from a nozzle to the surface container containing the biological sample in cryopreservative. Inside a freezing chamber, containers are placed on racks that are located proximal to a sprayrail containing nozzles. Ice seeding is performed by discharge of liquefied or chilled gas from the nozzles to the container surface at the solid-liquid equilibrium temperature of the cryopreservative. Localized cooling of the container surface by the discharge results in ice seed formation within the container.

Inventors: Cosman; Maury D. (Woburn, MA)

Assignee: Organogenesis Inc.

International Classification: A01N 1/02 (20060101); F25D 29/00 (20060101); F25D 3/10 (20060101); F25D 25/00 (20060101); G01N 1/42 (20060101); F24F 003/16 ()

Expiration Date: 01/02/2018