Patent Number: 6,168,928

Title: Modification of pertussis toxin

Abstract: The three-dimensional structure of crystalline pertussis holotoxin (PT) has been determined by X-ray crystallography. Crystal structures have also been determined for complexes of pertussis toxin with molecules relevant to the biological activity of PT. These three-dimensional structures were analyzed to identify functional amino acids appropriate for modification to alter the biological properties of PT. Similar procedures may be used to predict amino acids which contribute to the toxicity of the holotoxin, to produce immunoprotective, genetically-detoxified analogs of pertussis toxin.

Inventors: Read; Randy J. (Edmonton, CA), Stein; Penelope E. (Cambridge, GB), Cockle; Stephen A. (Richmond Hill, CA), Oomen; Raymond P. (Tottenham, CA), Loosmore; Sheena (Aurora, CA), Klein; Michel H. (Willowdale, CA), Armstrong; Glen D. (Edmonton, CA), Hazes; Bart (Edmonton, CA)

Assignee: Connaught Laboratories Limited

International Classification: C07K 14/195 (20060101); C07K 14/235 (20060101); G01N 33/566 (20060101); G01N 33/68 (20060101); C12N 009/10 (); C12N 009/14 (); C12N 015/09 (); C12Q 001/48 (); C12Q 001/34 ()

Expiration Date: 01/02/2018