Patent Number: 6,200,762

Title: Photon reducing agents and compositions for fluorescence assays

Abstract: The present invention provides a method for reducing undesirable light emission from a sample using at least one photon producing agent and at least one photon reducing agent (e.g. dye-based photon reducing agents). The present invention further provides a method for reducing undesirable light emission from a sample (e.g., a biochemical or cellular sample) with at least one photon producing agent and at least one collisional quencher. The present invention also provides a method for reducing undesirable light emission from a sample (e.g., a biochemical or cellular sample) with at least one photon producing agent and at least one quencher, such as an electronic quencher. The present invention further provides a method of determining bound and free analyte in a sample using at least one photon reducing agent. The present invention also provides a method of screening test chemicals in fluorescent assays using photon reducing agents. The present invention also provides compositions and kits for practicing these methods.

Inventors: Zlokarnik; Gregor (San Diego, CA), Negulescu; Paul (Solana Beach, CA), Knapp; Tom (Encinitas, CA), Tsien; Roger Y. (La Jolla, CA), Rink; Tim (La Jolla, CA)

Assignee: Aurora Biosciences Corporation

International Classification: C12Q 1/68 (20060101); G01N 33/536 (20060101); G01N 33/542 (20060101); G01N 33/50 (20060101); C12Q 001/70 (); C12Q 001/02 (); C12Q 001/34 (); G01N 033/542 (); B01L 003/00 ()

Expiration Date: 03/13/2018