Patent Number: 6,200,773

Title: Diagnostics based on tetrazolium compounds

Abstract: A reagent is suitable for measuring the concentration of an analyte in a hemoglobin-containing biological fluid, such as whole blood. The reagent comprises dehydrogenase enzyme that has specificity for the analyte, NAD, an NAD derivative, pyrrolo-quinoline quinone (PQQ), or a PQQ derivative, a tetrazolium dye precursor, a diaphorase enzyme or an analog thereof, and a nitrite salt. The reagent causes dye formation that is a measure of the analyte concentration. The nitrite salt suppresses interfering dye formation caused non-enzymatically by the hemoglobin. Preferably, the reagent is used in a dry strip for measuring ketone bodies, such as beta-hydroxybutyrate.

Inventors: Ouyang; Tianmei (Milpitas, CA), Yu; Yeung Siu (Pleasanton, CA)

Assignee: Lifescan, Inc.

International Classification: C12Q 1/32 (20060101); G01N 33/52 (20060101); G01N 33/64 (20060101); C12Q 001/32 (); C12M 001/40 ()

Expiration Date: 03/13/2018