Patent Number: 6,251,247

Title: Detection of degradation of RNA using microchannel electrophoresis

Abstract: Degradation of RNA present in a sample is detected by using, as an indicator, rRNA included in the RNA. The steps include: (a) introducing the sample onto a microchannel, at an introducing point, filled with an electrophoresis separation gel; (b) conducting electrophoresis of rRNA fragments present in the sample to force rRNA fragments to migrate through the electrophoresis separation gel; (c) detecting rRNA fragments passing through a detection point located downstream of the introducing point, to obtain detection patterns; and (d) determining degradation of RNA present in the sample based on the detection patterns of rRNA. A sample containing degraded mRNA can also be screened by detecting rRNA degradation in the above microchannel electrophoresis.

Inventors: Mitsuhashi; Masato (Irvine, CA), Ogura; Mieko (Irvine, CA), Watanabe; Kenji (Yuki, JP), Agata; Yoichi (Makabe-machi, JP)

Assignee: Hitachi Chemical Co., Ltd.

International Classification: G01N 27/447 (20060101); G01N 027/26 (); G01N 027/447 ()

Expiration Date: 06/26/2018