Patent Number: 6,251,384

Title: Metastasis models using green fluorescent protein (GFP) as a marker

Abstract: A method to follow the progression of metastasis of a primary tumor, which method comprises removing fresh organ tissues from a vertebrate subject which has been modified to contain tumor cells that express GFP and observing the excised tissues for the presence of fluorescence is disclosed. The fluorescence can also be monitored by observing the tissues in situ. Vertebrate subjects which contain GFP producing tumors are useful models to study the mechanism of metastasis, as well as to evaluate candidate protocols and drugs. In addition, subjects already harboring tumors can be treated so as to modify the endogenous tumors to contain GFP. This permits clinical applications. Finally, by injecting a contrast dye into a subject harboring a GFP-labeled tumor, angiogenesis in the tumor can be observed directly.

Inventors: Tan; Yuying (San Diego, CA), Chishima; Takashi (Yokohama, JP)

Assignee: AntiCancer, Inc.

International Classification: A01K 67/027 (20060101); A61K 49/00 (20060101); C07K 14/435 (20060101); A61K 48/00 (20060101); A61K 035/00 (); A61K 048/00 (); C12N 015/63 (); C12N 015/85 ()

Expiration Date: 06/26/2018