Patent Number: 6,251,390

Title: Purified chitinases and use thereof

Abstract: Two chitinases from Trichoderma harzianum P1 (ATCC 74058) show chitin-containing-fungus-inhibiting activity. One is an endochitinase and the other is a chitobiase. Both have molecular weights of 40 kDa and isoelectric points of 3.9. A .lambda.gtll recombinant encoding for the endochitinase has been prepared (ATCC 55338) and the gene encoding for the endochitinase has been removed from the DNA of the recombinant by the restriction enzyme, Not I. Endochitinases and chitobiases including the two purified from Trichoderma harzianum strain P1 demonstrate synergy with each other in anti-fungal effect.

Inventors: Harman; Gary E. (Geneva, NY), Broadway; Roxanne M. (Geneva, NY), Tronsmo; Arne (Aas, NO), Lorito; Matteo (Geneva, NY), Hayes; Christopher K. (Geneva, NY), Di Pietro; Antonio (Binningen, CH)

Assignee: Cornell Research Foundation, Inc.

International Classification: C12N 9/24 (20060101); A61K 038/47 (); C12N 009/42 ()

Expiration Date: 06/26/2018