Patent Number: 6,251,398

Title: Materials and methods for intracellular transport and their uses

Abstract: Coupled polypeptides and fusion polypeptides for intracellular transport, and their preparation and use, include (i) an aminoacid sequence with the transport function of herpesviral VP22 protein (or a homologue, e.g. from VZV, BHV or MDV) and (ii) another protein sequence selected from (a) proteins for cell cycle control; (b) suicide proteins; (c) antigenic sequences or antigenic proteins from microbial and viral antigens and tumor antigens; (d) immunomodulating proteins; and (e) therapeutic proteins. The coupled proteins can be used for intracellular delivery of protein sequences (ii), to exert the corresponding effector function in the target cell, and the fusion polypeptides can be expressed from corresponding polynucleotides, vectors and host cells.

Inventors: O'Hare; Peter Francis Joseph (Oxted, GB), Elliott; Gillian Daphne (Oxted, GB)

Assignee: Marie Curie Cancer Care

International Classification: C07K 14/005 (20060101); C07K 14/035 (20060101); A61K 039/00 (); C12N 015/62 (); C07K 019/00 ()

Expiration Date: 06/26/2018