Patent Number: 6,251,467

Title: Isolation of cellular material under microscopic visualization

Abstract: A method of microdissection which involves forming an image field of cells of the tissue sample utilizing a microscope, identifying at least one zone of cells of interest from the image field of cells which at least one zone of cells of interest includes different types of cells than adjacent zones of cells, and extracting the at least one zone of cells of interest from the tissue sample. The extraction is achieved by contacting the tissue sample with a transfer surface that can be selectively activated so that regions thereof adhere to the zone of cells of interest to be extracted. The transfer surface includes a selectively activatable adhesive layer which provides, for example, chemical or electrostatic adherence to the selected regions of the tissue sample. After the transfer surface is activated, the transfer surface and tissue sample are separated. During separation, the zone of cells of interest remains adhered to the transfer surface and is thus separated from the tissue sample, the zone of cells of interest may then be molecularly analyzed.

Inventors: Liotta; Lance A. (Potomac, MD), Emmert-Buck; Michael (Silver Spring, MD), Krizman; David B. (Gaithersburg, MD), Chuaqui; Rodrigo (Las Condes, CL), Linehan; W. Marston (North Bethesda, MD), Trent; Jeffry M. (Rockville, MD), Bonner; Robert F. (Washington, DC), Goldstein; Seth R. (Bethesda, MD), Smith; Paul D. (Annapolis, MD), Peterson; John I. (Falls Church, VA)

Assignee: The United States of America as represented by the Department of Health and Human Services

International Classification: C12M 1/26 (20060101); C12M 1/34 (20060101); G01N 1/28 (20060101); G02B 21/32 (20060101); G01N 33/50 (20060101); G01N 1/04 (20060101); G01N 1/30 (20060101); G01N 1/31 (20060101); G01N 1/02 (20060101); G01N 1/08 (20060101); G01N 35/00 (20060101); B05D 001/00 ()

Expiration Date: 06/26/2018