Patent Number: 6,251,584

Title: Specific binding assays using methyl orange

Abstract: The present invention relates to a method for improving the specificity of a specific binding assay by adding methyl orange to a coating solution in an amount sufficient for improving specificity of an immunoassay conducted using said coating. The present invention also relates to a method for the detection of antibodies to hepatitis C virus is performed by, i) providing a solid phase comprising a coating solution comprising methyl orange and at least one first binding ligand for antibodies to hepatitis C virus; ii) contacting the solid phase with a sample that may contain antibodies to hepatitis C virus; iii) contacting the solid phase with at least one second binding ligand for antibodies to hepatitis C virus, said second ligand labelled directly or indirectly with a detectable group and iv) measuring the amount of the detectable group bound to the solid phase.

Inventors: Unadkat; Pratul (Serangoon Central, SG)

Assignee: Ortho-Clinical Diagnostics

International Classification: G01N 33/576 (20060101); G01N 33/543 (20060101); C12Q 001/70 (); G01N 033/53 (); G01N 033/566 (); G01N 033/532 ()

Expiration Date: 06/26/2018