Patent Number: 6,251,591

Title: Quantitative method for detecting nucleotide concentration

Abstract: The invention provides a method for rapidly, economically and efficiently determining the concentration of a target nucleobase-containing sequence in a fluid medium using laser induced fluorescence of antisense probes. When hybridization complexes and unhybridized probes are separated prior to detection, the fluorescent intensity of the test medium is proportional to the concentration of the target sequence. When hybridization complexes and unhybridized probes are not separated prior to detection, the fluorescent intensity of the test medium is inversely proportional to the concentration of the target sequence. The method can be used to determine the concentration of a contaminant in a sample as a part of a system of quality control.

Inventors: Wu; Yuan Min (Thornhill, CA), Nie; Eileen Xiao-Feng (Thornhill, CA)

Assignee: Lorne Park Research, Inc.

International Classification: C12P 19/00 (20060101); C12P 19/34 (20060101); C12Q 1/68 (20060101); C12Q 001/68 (); C12P 019/34 ()

Expiration Date: 06/26/2018