Patent Number: 6,251,593

Title: Identification of eukaryotic growth-related genes and promoter isolation vector and method of use

Abstract: A polynucleotide encoding chitin synthase (CHS1), an enzyme essential for cell wall synthesis and yeast cell growth, is provided. A maltose responsive promoter (MRP) isolated using the promoter library of the invention is also described. The present invention also provides a vector for isolation of a eukaryotic regulatory polynucleotide, i.e., promoter. The vector is useful in the method of the invention which comprises identifying a eukaryotic regulatory polynucleotide, i.e., promoter region, by complementing the growth of an auxotrophic host cell containing the vector of the invention, which includes a promoter region operably linked to a promoterless auxotrophic gene. The vector is introduced into the host cell chromosome by targeted integration. Also provided is a library containing host cells having the vector of the invention integrated in the chromosome of the host cell.

Inventors: Koltin; Yigal (Newton, MA), Riggle; Perry (Norwood, MA), Gavrias; Vicky (Watertown, MA), Bulawa; Chris (Arlington, MA), Winter; Kenneth R. (Seattle, WA)

Assignee: Millennium Pharmaceuticals, Inc.

International Classification: C12N 15/67 (20060101); C12N 9/10 (20060101); C12Q 001/68 (); C12N 001/14 (); C12N 015/63 (); C07H 021/04 ()

Expiration Date: 06/26/2018