Patent Number: 6,251,594

Title: Cancer diagnostic method based upon DNA methylation differences

Abstract: There is disclosed a cancer diagnostic method based upon DNA methylation differences at specific CpG sites. Specifically, the inventive method provides for a bisulfite treatment of DNA, followed by methylation-sensitive single nucleotide primer extension (Ms-SNuPE), for determination of strand-specific methylation status at cytosine residues.

Inventors: Gonzalgo; Mark L. (South Pasadena, CA), Jones; Peter A. (La Canada, CA), Liang; Gangning (Rowland Heights, CA)

Assignee: USC/Norris Comprehensive Cancer Ctr.

International Classification: C07H 21/04 (20060101); C07H 21/00 (20060101); C12P 19/00 (20060101); C12P 19/34 (20060101); C12Q 1/68 (20060101); C12Q 001/68 (); C12P 019/34 (); C07H 021/04 ()

Expiration Date: 06/26/2018