Patent Number: 6,251,601

Title: Simultaneous measurement of gene expression and genomic abnormalities using nucleic acid microarrays

Abstract: The invention comprises a multi-color, comparative hybridization assay method using an array of nucleic acid target elements attached to a solid support for the simultaneous detection of both gene expression and chromosomal abnormalities in a tissue sample. The method of the invention employs a comparative hybridization of a tissue mRNA or cDNA sample labeled in a first fluorescent color, a tissue chromosomal DNA sample labeled in a second fluorescent color, and at least one reference nucleic acid labeled in a third fluorescent color, to the array. The fluorescent color presence and intensity at each of at least two target elements are detected and the fluorescent ratios (i) of the first and third colors and (ii) the second and third colors determined. Gene expression and chromosomal abnormalities are thus simultaneously detected.

Inventors: Bao; Yijia (Naperville, IL), Che; Diping (Westmont, IL), Li; Wan-Liang (Lisle, IL), Muller; Uwe Richard (Plano, IL), Seelig; Steven A. (Naperville, IL), Shi; Jufang (Hinsdale, IL)

Assignee: Vysis, Inc.

International Classification: C12Q 1/68 (20060101); C12Q 001/68 ()

Expiration Date: 06/26/2018