Patent Number: 6,251,602

Title: Cell systems having specific interaction of peptide binding pairs

Abstract: This invention relates to novel modified host cells which express heterologous fused proteins and methods of screening for test samples having peptide-binding activity; wherein the modified host cell comprises: (a) a gene sequence encoding a heterologous fusion protein; said fusion protein comprising a first peptide of a peptide binding pair, or segment of said first peptide, which is joined to either a DNA binding domain or its corresponding transcriptional activation domain of a transcriptional activation protein; (b) a gene sequence encoding a heterologous fusion protein, said fusion protein comprising a second peptide of the peptide binding pair in (a), or a segment thereof, fused to either a DNA binding domain or its corresponding transcriptional activation domain, whichever one is not employed in (a); (c) a reporter gene operatively associated with the transcriptional activation protein, or a portion thereof; (d) optionally, a deletion or mutation in the chromosomal DNA of the host cell for the transcriptional activation protein if present in the selected host cell.

Inventors: Young; Kathleen H. (Newtown, PA), Ozenberger; Bradley A. (Yardley, PA)

Assignee: American Cyanamid Company

International Classification: C07K 14/435 (20060101); C07K 14/61 (20060101); C07K 14/72 (20060101); C12Q 1/68 (20060101); C12Q 001/68 (); C12N 001/19 ()

Expiration Date: 06/26/2018