Patent Number:
6,251,607
Title:
PCR primers for the rapid and specific detection of Salmonella typhimurium
Abstract:
The invention provides a set of two PCR primers designed based on a DNA sequence of a gene encoding malic acid dehydrogenase and a specific DNA of Salmonella typhimurium. The invention provides also a DNA probe specific for the above-mentioned PCR primers. Finally, a PCR method using above-mentioned primers is provided for the rapid and specific detection of Salmonella typhimurium in food and clinical specimens such as human fecal specimens. Said PCR method comprises further a Southern hybridization assay for detecting PCR products. The whole process could be shortened from 5-7 days for BAM method to 1-2 days.
Inventors:
Tsen; Hau-Yang (Taichung, TW), Lin; Jer-Sheng (Taichung, TW)
Assignee:
National Science Council of Republic of China
International Classification:
C12Q 1/68 (20060101); C12Q 001/68 (); C12P 019/34 (); C07H 021/04 ()
Expiration Date:
06/26/2018