Patent Number: 6,251,607

Title: PCR primers for the rapid and specific detection of Salmonella typhimurium

Abstract: The invention provides a set of two PCR primers designed based on a DNA sequence of a gene encoding malic acid dehydrogenase and a specific DNA of Salmonella typhimurium. The invention provides also a DNA probe specific for the above-mentioned PCR primers. Finally, a PCR method using above-mentioned primers is provided for the rapid and specific detection of Salmonella typhimurium in food and clinical specimens such as human fecal specimens. Said PCR method comprises further a Southern hybridization assay for detecting PCR products. The whole process could be shortened from 5-7 days for BAM method to 1-2 days.

Inventors: Tsen; Hau-Yang (Taichung, TW), Lin; Jer-Sheng (Taichung, TW)

Assignee: National Science Council of Republic of China

International Classification: C12Q 1/68 (20060101); C12Q 001/68 (); C12P 019/34 (); C07H 021/04 ()

Expiration Date: 06/26/2018