Patent Number: 6,251,614

Title: Rapid methods for identifying modifiers of cellular apoptosis activity

Abstract: The invention provides a single-well, microscale method of determining the specific apoptotic activity of a cell. The method consists of contacting a cell population of about 1.times.10.sup.5 cells for a time period of between about 30 minutes and 4 hours with a sufficient volume of medium containing an apoptotic specific diagnostic reagent and a diagnostic accessory reagent so as to cover the cell population, and determining the activity of the apoptotic specific diagnostic reagent. The invention also provides a method of identifying a compound which induces apoptosis. The invention further provides a rapid method of identifying a compound which inhibits apoptosis.

Inventors: Fritz; Lawrence C. (Rancho Santa Fe, CA), Diaz; Jose-Luis (Carlsbad, CA), Armstrong; Robert C. (San Diego, CA), Tomaselli; Kevin J. (San Diego, CA)

Assignee: Idun Pharmaceuticals, Inc.

International Classification: G01N 33/50 (20060101); C12Q 001/00 (); C12Q 001/02 (); C12Q 001/37 ()

Expiration Date: 06/26/2018