Patent Number: 6,251,623

Title: Quick assay method for the activity of a plant-derived, asparagine residue-specific endoprotease

Abstract: A quick assay method for the activity of a plant-derived, asparagine residue-specific endoprotease is disclosed which comprises measuring fluorescence generated by a fluorescence quenching substrate split by an asparagine residue-specific endoprotease. The fluorescence quenching substrate comprises an oligopeptide having in its amino acid sequence at least one asparagine residue, whose C-terminal side is other than an isoleucine residue, a leucine residue, or a valine residue, wherein the oligopeptide has a 7-methoxycoumarin-4-yl-acetyl group arranged on its N-terminal side and a 2,4-dinitrophenyl group arranged on its C-terminal side.

Inventors: Arahira; Masaomi (Tsukuba, JP), Fukazawa; Chikafusa (Tsukuba, JP)

Assignee: Director of National Food Research Institute, Ministry of Agriculture, Forestry and Fisheries

International Classification: C12Q 1/37 (20060101); C12Q 001/37 (); C12Q 001/00 (); C12N 009/00 ()

Expiration Date: 06/26/2018