Patent Number:
6,251,635
Title:
Recombinant preparation of calcitonin fragments and use thereof in the preparation of calcitonin and related analogs
Abstract:
A process for the recombinant preparation of a calcitonin fragment and the use of the fragment in the preparation of calcitonin and related analogs is provided. The process includes recombinantly forming a fusion protein which includes the calcitonin fragment linked to a carbonic anhydrase. The recombinantly formed fusion protein is subsequently cleaved to produce a polypeptide which includes the calcitonin fragment. A method for producing a calcitonin carba analog which includes condensing a desaminononapeptide with the recombinantly formed calcitonin fragment is also provided.
Inventors:
Wagner; Fred W. (Walton, NE), Stout; Jay S. (Lincoln, NE), Henriksen; Dennis B. (Lincoln, NE), Partridge; Bruce E. (Lincoln, NE), Holmquist; Bart (Lincoln, NE), Frank; Julie A. (Lincoln, NE)
Assignee:
Bionebraska, Inc.
International Classification:
C07K 14/435 (20060101); C07K 14/585 (20060101); C12N 9/88 (20060101); C12P 021/04 (); C12P 021/06 (); C12N 001/20 (); C12N 015/00 (); A61K 038/00 ()
Expiration Date:
06/26/2018