Patent Number: 6,251,637

Title: Use of extremely thermophilic DNA-polymerases

Abstract: Cycle sequencing of DNA with marker-labeled dideoxynucleotides is performed using extremely thermophilic DNA polymerases in a one-lane technique in which dideoxynucleotides labeled with a dye as a marker are used to obtain sequence ladders of more 500 bases. Chain elongation is performed at a temperature of 65 to C. Surprisingly, it was found that chain elongation temperatures of substantially more than C. could be used in cycle sequencing of DNA with marker labeled dye dideoxynucleotides when using extremely thermophilic DNA polymerases.

Inventors: Blusch; Juergen (Munich, DE)

Assignee: GSF Forschungszentrum fuer Umwelt und Gesundheit GmbH

International Classification: C12Q 1/68 (20060101); C12N 9/12 (20060101); C12Q 001/68 (); C12P 019/34 (); C07H 021/02 (); C12N 015/00 ()

Expiration Date: 06/26/2018