Patent Number: 6,251,639

Title: Methods and compositions for linear isothermal amplification of polynucleotide sequences, using a RNA-DNA composite primer

Abstract: The present invention provides novel isothermal, single primer linear nucleic acid amplification methods. Methods for amplifying complementary DNA using a composite primer, primer extension, strand displacement, and optionally a termination sequence, are provided. Methods for amplifying sense RNA using a composite primer, primer extension, strand displacement, optionally template switching, a propromoter oligonucleotide and transcription are also provided. The invention further provides compositions and kits for practicing said methods, as well as methods which use the amplification products.

Inventors: Kurn; Nurith (Palo Alto, CA)

Assignee: Nugen Technologies, Inc.

International Classification: C12Q 1/68 (20060101); C12Q 001/68 (); C12P 019/34 (); C07H 021/02 (); C07H 021/04 (); G01N 033/53 ()

Expiration Date: 06/26/2018