Patent Number: 6,251,650

Title: Pseudomonas putida amidase polypeptide useful for the production of chiral amides and acids

Abstract: The present invention provides a nitrile hydratase nucleic acid fragment isolated from Pseudomonas putida which encodes a nitrile hydratase activity capable of catalyzing the hydrolysis of certain racemic nitriles to the corresponding R- or S-amides. Also provided are transformed microorganisms capable of the active expression of said nitrile hydratase activity. Additionally, the invention provides a transformant harboring the nitrile hydratase gene in conjunction with an amidase gene, both of which may be co-expressed producing active nitrile hydratase and amidase enzymes respectively. Methods for the production of such enantiomeric materials are also provided.

Inventors: Fallon; Robert Donald (Elkton, MD), Nelson; Mark James (Newark, DE), Payne; Mark Scott (Wilmington, DE)

Assignee: E. I. du Pont de Nemours and Company

International Classification: C12P 13/00 (20060101); C12P 7/40 (20060101); C12P 17/00 (20060101); C12P 13/02 (20060101); C12N 9/80 (20060101); C12P 41/00 (20060101); C12N 9/78 (20060101); C12N 009/78 ()

Expiration Date: 06/26/2018