Patent Number: 6,251,651

Title: Protein-deamidating enzyme, gene encoding the same, production process therefor, and use thereof

Abstract: A novel enzyme which has an activity to release side chain carboxyl groups and ammonia from a protein by acting upon side chain amido groups in the protein. This invention relates to a method for the production of an enzyme, which comprises culturing in a medium a strain that belongs to a bacterium classified into Cytophagales or Actinomycetes and has the ability to produce an enzyme having a property to deamidate amido groups in protein, thereby effecting production of said enzyme, and subsequently collecting said enzyme from the culture mixture. It also relates to a method for the modification of protein making use of a novel enzyme which directly acts upon amido groups in protein as well as to an enzyme which has a property to deamidate amido groups in protein and a gene which encodes said enzyme.

Inventors: Yamaguchi; Shotaro (Norwich, GB), Matsuura; Akira (Aichi, JP)

Assignee: Amano Pharmaceutical Co., Ltd.

International Classification: C12N 9/80 (20060101); C12N 9/78 (20060101); C12N 009/00 (); C12N 009/50 (); C12N 009/52 (); C12N 009/78 ()

Expiration Date: 06/26/2018