Patent Number: 6,251,665

Title: Directed maturation of stem cells and production of programmable antigen presenting dentritic cells therefrom

Abstract: A method is described whereby dendritic cells derived from the CD34+ and CD34- hematopoietic cell lineages are directed to become programmable antigen presenting cells. The programmed cells may be pulsed with tumor cell RNA or tumor cell RNA expression products. The protocol provides for directing the maturation of dendritic cells to become antigen presenting cells. The protocol further provides for isolating tumor cell RNA from biopsy material that has been prepared in paraffin block storage. The directed dendritic cell is provided with a plurality of tumor markers by using tumor RNA in toto, the poly A+RNA fraction or the expression product of such RNA. Once activated the dendritic cells are incubated with T4 and T8 lymphocytes to stimulate and sensitize the T lymphocytes which upon introduction either into a donor host or a nondonor recipient will provide immune response protection.

Inventors: Cezayirli; Cem (Birmingham, AL), Silvers; Mel (N. Miami Beach, FL)


International Classification: A61K 38/19 (20060101); A61K 38/20 (20060101); C12N 5/06 (20060101); C12N 005/02 (); C12N 005/06 (); C12N 005/08 (); C12N 014/52 (); C12N 014/525 ()

Expiration Date: 06/26/2018