Patent Number: 6,251,670

Title: Method of culturing cells in suspension using lectins

Abstract: An object of the present invention is to provide a method of enabling perfusion culture efficiently and simply by agglutinating cells with Lectin, which is a naturally-occurring agglutinin, thereby separating the cells and the culture medium. According to the method of the present invention, lectin is added to a culture medium to quickly agglutinate and precipitate the cells, thereby separating the culture medium and the cells. Hence, it is easy to remove old culture medium and replenish with fresh culture medium. Accordingly, if the method of the present invention is used, the perfusion culture is performed automatically and on an industrial scale.

Inventors: Yoshimoto; Tanihiro (Kanazawa, JP), Takamatsu; Hiroyuki (Kanazawa, JP)

Assignee: President of Kanazawa University

International Classification: C12N 5/16 (20060101); C12N 005/00 ()

Expiration Date: 06/26/2018