Patent Number: 6,251,672

Title: Culturing mammalian cells in contact with cell surface proteins

Abstract: Mammalian cells which require contact with cell surface proteins for activation, differentiation and/or proliferation are cultured in contact with an immobilized mammalian cell, with an immobilized membrane vesicle from a mammalian cell or with immobilized membrane vesicles from at least two mammalian cell populations. The mammalian cell or membrane vesicle can be modified with a first partner of a biological binding pair, and immobilized on a solid carrier via a second partner of the biological binding pair. Culturing can be with a first immobilized membrane vesicle and subsequently with a second immobilized membrane vesicle. The membrane vesicle contains part of the natural surface of a mammalian cell that includes a signal triggering surface protein. The membrane vesicle can be immobilized via a binding partner of a cell surface molecule.

Inventors: Kubbies; Manfred (Penzberg, DE), Dormer; Peter (Gilching, DE), Meissner; Petra (Julich, DE)

Assignee: GSF-Forschungszentrum fur Umwelt und Gesundheit

International Classification: C12N 5/00 (20060101); C12N 005/00 (); C12N 011/00 (); C12N 011/02 (); C12N 011/06 (); C12N 005/06 ()

Expiration Date: 06/26/2018