Patent Number: 6,251,674

Title: Evolution of whole cells and organisms by recursive sequence recombination

Abstract: The invention provides methods employing iterative cycles of recombination and selection/screening for evolution of whole cells and organisms toward acquisition of desired properties. Examples of such properties include enhanced recombinogenicity, genome copy number, and capacity for expression and/or secretion of proteins and secondary metabolites.

Inventors: Tobin; Matthew (San Jose, CA), Stemmer; William P. C. (Los Gatos, CA), Ness; Jon E. (Sunnyvale, CA), Minshull; Jeremy (Menlo Park, CA)

Assignee: Maxygen, Inc.

International Classification: C12N 15/82 (20060101); C12Q 1/68 (20060101); C12N 015/00 (); C12Q 001/68 (); C07H 021/02 (); C07H 021/04 ()

Expiration Date: 06/26/2018