Patent Number: 6,251,677

Title: Hybrid adenovirus-AAV virus and methods of use thereof

Abstract: The present invention provides a hybrid vector construct which comprises a portion of an adenovirus, 5' and 3' ITR sequences from an AAV, and a selected transgene. Also provided is a hybrid virus linked via a polycation conjugate to an AAV rep gene to form a single particle. These trans-infection particles are characterized by high titer transgene delivery to a host cell and the ability to stably integrate the transgene into the host cell chromosome. Also disclosed is the use of the hybrid vectors and viruses to produce large quantities of recombinant AAV.

Inventors: Wilson; James M. (Gladwyne, PA), Kelley; William M. (Ann Arbor, MI), Fisher; Krishna J. (New Orleans, LA)

Assignee: The Trustees of the University of Pennsylvania

International Classification: C12N 7/00 (20060101); C12N 15/864 (20060101); C12N 15/861 (20060101); A61K 48/00 (20060101); C12N 015/861 (); C12N 015/864 (); C12N 005/10 (); C12N 015/63 (); A61K 048/00 ()

Expiration Date: 06/26/2018