Patent Number: 6,252,054

Title: Immobilization of SH group-containing compounds in the presence of a sulfite-containing antioxidant

Abstract: Immobilization of SH group-containing compounds on a solvent-insoluble support is carried out in the presence of an antioxidant to prevent oxidation of SH groups to S--S bonds. This improves immobilization efficiency and suppresses deterioration of inherent characteristics of the SH group-containing compound. Antioxidants include sodium pyrosulfite (sodium disulfite), sodium sulfite, sodium hydrogensulfite, sodium hydrosulfite and L-ascorbic acid. SH group-containing compounds include cysteine, peptides or proteins containing cysteine and thiol compounds such as ethanethiol, aminoethanethiol, benzylthiol and thiophenol. Preferably, the SH group-containing compound has a molecular weight not more than 3.times.10.sup.4. The support may be activated by a functional group such as glycidyl, imidocarbonato, tosyl, tresyl, carboxyl, amino, azido or hydroxyl. The support can be inorganic such as glass beads or organic such as a synthetic polymer or a polysaccharide.

Inventors: Ogino; Eiji (Kobe, JP), Nishimoto; Takehiro (Osaka, JP), Nomura; Michio (Kakogawa, JP)

Assignee: Kaneka Corporation

International Classification: C07K 17/02 (20060101); C07K 17/00 (20060101); G01N 33/543 (20060101); C07K 017/02 (); C07K 017/14 (); C12N 011/16 (); G01N 033/551 (); G01N 033/544 ()

Expiration Date: 06/26/2018