Patent Number: 6,252,130

Title: Production of somatic mosaicism in mammals using a recombinatorial substrate

Abstract: The present invention relates to recombinatorial substrates which include a promoter, a terminator, a gene positioned 3' to the terminator and whose expression is to be controlled, and recombination sites on each side of the terminator such that when the substrate is treated with a specific recombinase the gene will be expressed. Recombinatorial substrates which have a promoter, a gene to be controlled, and recombination sites on each side of the gene which when treated with recombinase delete the gene are also provided. Also enclosed are methods of creating transgenic mammals carrying the recombinatorial substrate and methods for activating the recombinatorial substrate.

Inventors: Federoff; Howard (Rochester, NY)

Assignee: University of Rochester

International Classification: A01K 67/027 (20060101); C07K 14/435 (20060101); C07K 14/48 (20060101); C12N 9/00 (20060101); C12N 15/869 (20060101); C12N 15/63 (20060101); C12N 15/85 (20060101); C12N 015/09 (); C12N 015/00 (); C12N 015/63 (); C12N 005/00 ()

Expiration Date: 06/26/2018