Patent Number: 6,252,132

Title: Mutant mouse containing a knockout mutation in DNA encoding an endogenous alpha 4 subunit of nicotinic acetylcholine receptor

Abstract: A mutant mouse has germ cells and somatic cells containing a mutation comprising a disruption of the endogenous .alpha.4 subunit of the nicotinic acetylcholine receptor (nAChR) gene, wherein the disrupted .alpha.4 subunit of the nAChR gene results in the mouse lacking detectable levels of the endogenous .alpha.4 subunit of nAChR without a change in level of expression of other nAChR subunits as compared to a wild type mouse. The mutant mouse is useful for studying the roles of the various subunits of the nAChR. The results are useful in studying the antinociceptive, hypothermia, and locomotor effects of nicotine in other mammals.

Inventors: Changeux; Jean-Pierre (Paris, FR), Marubio; Lisa (Paris, FR)

Assignee: Institut Pasteur

International Classification: C07K 14/435 (20060101); C07K 14/705 (20060101); C12N 15/85 (20060101); A01K 067/027 (); A01K 067/00 (); A01K 067/033 (); C12N 015/00 ()

Expiration Date: 06/26/2018