Patent Number: 6,254,754

Title: Chip for performing an electrophoretic separation of molecules and method using same

Abstract: A chip for performing an electrophoretic separation of micromolecular or macromolecular structures in two dimensions comprises a base substrate comprising a main surface. A channel is formed in the main surface of said base substrate in a first direction, wherein a first voltage is applicable across the channel in order to allow a separation of micromolecular structures or macromolecular structures in the first direction on the basis of a first separation mechanism. In addition, a two-dimensional separation matrix is formed in the main surface of the base substrate, wherein the separation matrix comprises a shallow recess and an array of stand-alone posts arranged in the recess. The recess extends perpendicular from the channel in a second direction, wherein a second voltage is applicable across the separation matrix in order to allow a separation of the structures on the basis of a second separation mechanism.

Inventors: Ross; Gordon (Karlsruhe, DE), Kaltenbach; Patrick (Bischweier, DE)

Assignee: Agilent Technologies, Inc.

International Classification: G01N 27/447 (20060101); G01N 027/26 (); G01N 027/447 ()

Expiration Date: 07/03/2018