Patent Number: 6,254,874

Title: Attenuated auxotrophic microorganisms having a combination of non-attenuating mutations and method for making same

Abstract: A live non-virulent vaccine composition and method for preparing the same comprising a virulent microorganismal strain which contains at least two mutations, wherein the first mutation results in an auxotrophic mutant which requires for proliferation, a nutrient which is normally available in the host tissues in an amount required by the auxotrophic mutant for proliferation and the second mutation results in the inability of the auxotrophic mutant to specifically transport the required nutrient from host-tissues into the auxotrophic mutant thereby producing an attenuated strain.

Inventors: Mekalanos; John J. (Cambridge, MA), Klose; Karl E. (Newton, MA)

Assignee: President and Fellows of Harvard College

International Classification: C07K 14/195 (20060101); C07K 14/255 (20060101); C12N 9/06 (20060101); A61K 039/00 (); A61K 039/02 (); A61K 039/112 (); A01N 063/00 ()

Expiration Date: 07/03/2018