Patent Number: 6,255,113

Title: Homologous sequence targeting in eukaryotic cells

Abstract: The invention relates to methods for targeting an exogenous polynucleotide or exogenous complementary polynucleotide pair to a predetermined endogenous DNA target sequence in a eukaryotic cell by homologous pairing, particularly for altering an endogenous DNA sequence, such as a chromosomal DNA sequence, typically by targeted homologous recombination. In certain embodiments, the invention relates to methods for targeting an exogenous polynucleotide having a linked chemical substituent to a predetermined endogenous DNA sequence in a metabolically active eukaryotic cell, generating a DNA sequence-specific targeting of one or more chemical substituents in an intact nucleus of a metabolically active eukaryotic cell, generally for purposes of altering a predetermined endogenous DNA sequence in the cell. The invention also relates to compositions that contain exogenous targeting polynucleotides, complementary pairs of exogenous targeting polynucleotides, chemical substituents of such polynucleotides, and recombinase proteins used in the methods of the invention.

Inventors: Zarling; David A. (Menlo Park, CA), Sena; Elissa P. (Palo Alto, CA)

Assignee: SRI International

International Classification: C07K 14/435 (20060101); C07K 14/82 (20060101); C07K 14/47 (20060101); C12N 15/87 (20060101); C12N 15/90 (20060101); A61K 38/00 (20060101); A61K 48/00 (20060101); C12N 015/63 (); C12N 015/00 (); C12N 015/09 (); C12N 005/00 ()

Expiration Date: 07/03/2018