Patent Number: 6,294,711

Title: Gene expression in plants

Abstract: The invention provides chimeric genes comprising a first promoter recognized by DNA-dependent RNA polymerase different from a eukaryotic RNA polymerase II; a DNA region encoding a chimeric RNA comprising a 5' UTR, an AU-rich heterologous coding sequence, a 3' UTR; and optionally a terminator sequence recognized by said RNA polymerase, wherein the first promoter and the DNA region encoding the chimeric RNA are operably linked such that upon transcription by the RNA polymerase an uncapped RNA species is produced which comprises a first translation enhancing sequence derived from the 5' region of genomic or subgenomic RNA of a positive stranded RNA plant virus; a heterologous RNA coding sequence encoding a polypeptide or protein of interest, preferably from an AT-rich gene; and a second translation enhancing sequence derived from the 3' region of gonomic or subgenomic RNA of a positive-stranded RNA plant virus, wherein the uncapped RNA species is capable of being translated in the cytoplasm of a plant cell to produce the protein or polypeptide. Also provided are plant cells and plants comprising these chimeric genes, integrated in their nuclear DNA, whereby the plant cell produces the RNA polymerases corresponding to the used promoters and terminators. Further the invention provides a process for producing a plant expressing a protein or polypeptide encoded by a hoterologous gene which comprises the steps of transforming the nuclear genome of a plant cell with the above-mentioned chimeric genes; and regenerating a transformed plant from the transformed cell.

Inventors: Meulewaeter; Frank (Lokeren, BE), Cornelissen; Marcus (Heusden, BE), Van Aarssen; Roel (Nazareth, BE), Soetaert; Piet (Laarne, BE), Gossele; Veronique (Gent, BE)

Assignee: Plant Genetic Systems

International Classification: C12N 15/82 (20060101); C12N 005/04 (); C12N 015/82 (); C12N 015/90 (); A01H 005/00 ()

Expiration Date: 09/25/2018