Patent Number: 6,300,099

Title: Methods for producing secreted ligand-binding fusion proteins

Abstract: Methods for producing secreted receptor analogs and biologically active peptide dimers are disclosed. The methods for producing secreted receptor analogs and biologically active peptide dimers utilize a DNA sequence encoding a receptor analog or a peptide requiring dimerization for biological activity joined to a dimerizing protein. The receptor analog includes a ligand-binding domain. Polypeptides comprising essentially the extracellular domain of a human PDGF receptor fused to dimerizing proteins, the portion being capable of binding human PDGF or an isoform thereof, are also disclosed. The polypeptides may be used within methods for determining the presence of and for purifying human PDGF or isoforms thereof.

Inventors: Sledziewski; Andrzej Z. (Seattle, WA), Bell; Lillian Anne (Seattle, WA), Kindsvogel; Wayne R. (Seattle, WA)

Assignee: ZymoGenetics, Inc.

International Classification: C07K 14/435 (20060101); C07K 14/49 (20060101); C07K 19/00 (20060101); C07K 14/705 (20060101); C07K 14/71 (20060101); C07K 16/46 (20060101); C12P 21/04 (20060101); C12N 15/62 (20060101); C12N 15/09 (20060101); C12N 15/81 (20060101); C12N 015/09 (); C12N 015/62 (); C12N 015/81 (); C07K 016/46 (); C07K 014/49 ()

Expiration Date: 10/09/2018