Patent Number: 6,307,121

Title: Bacteriophage-based transgenic fish for mutation detection

Abstract: The present invention provides transgenic fish whose somatic and germ cells contain a genomically integrated bacteriophage lambda-derived transgene construct. The transgene construct can include an excisable test nucleic acid sequence containing a heterologous mutation target nucleic acid sequence that is detectable via bioassay in a bacterial cell into which the test nucleic acid has been introduced. The frequency of mutations in the mutation target nucleic acid sequence following exposure of the transgenic fish to one or more potentially mutagenic agents can thus be evaluated.

Inventors: Winn; Richard N. (Athens, GA)

Assignee: The University of Georgia Research Foundation, Inc.

International Classification: C12N 15/85 (20060101); C12Q 1/68 (20060101); G01N 033/00 (); A01K 067/027 (); C12N 015/00 (); C07H 021/02 (); C07H 021/04 ()

Expiration Date: 10/23/2018