Patent Number: 6,307,123

Title: Methods and compositions for transgene identification

Abstract: The present invention provides methods and compositions for the identification of transgenic seeds. This is accomplished by use of screenable markers linked to aleurone-specific promoters. The screenable markers can be provided as gene fusions with selectable markers, allowing both selection and screening of transformants. The use of aleurone-specific promoters, which also direct expression in embryogenic tissues, allows efficient selection of transgenic cells and the screening of viable transgenic seeds, while avoiding the deleterious effects associated with constitutive expression of screenable marker genes. Screening of transgenic seeds avoids the need for growing and assaying of seeds for transgenes and allows implementation of automated seed screening techniques for the identification of transgenic seeds.

Inventors: Kriz; Alan L. (Gales Ferry, CT), Spencer; T. Michael (Mystic, CT)

Assignee: Dekalb Genetics Corporation

International Classification: C12N 15/82 (20060101); C12N 015/00 (); C12M 015/09 (); A01H 005/00 (); A01H 001/02 ()

Expiration Date: 10/23/2018