Patent Number: 6,307,127

Title: Transformation of guar

Abstract: The invention relates to a method for the transformation of legumes of the genus Cyamopsis, in particular Agrobacterium-mediated transformation of guar (Cyamopsis tetragonoloba), by introducing a recombinant DNA sequence into at least one cell or protoplast and generating genetically modified explants using at least one selection or shoot growth medium comprising at least one compound selected from an auxin inhibitor, e.g. 2-(p-chlorophenoxy)-2-methylpropionic acid (PCIB), a .beta.-lactamase inhibitor, e.g. sulbactam, and an ethylene inhibitor, e.g. silver thiosulfate, so as to obtain genetically modified plant or part thereof containing in its genome at least one recombinant DNA sequence; to genetically modified plants produced by the method; and to the use of substances such as the .beta.-lactamase inhibitor sulbactam to facilitate transformation of guar and other plants.

Inventors: J.o slashed.rsboe; Morten (Nyk.o F, DK), Okkels; Finn T. (Roskilde, DK)

Assignee: Danisco A/S

International Classification: A01H 5/10 (20060101); C12N 15/82 (20060101); C12N 005/04 (); C12N 015/84 (); C12N 015/90 (); A01H 005/00 ()

Expiration Date: 10/23/2018